On the Feasibility of Social Network-based Pollution Sensing in ITSs

Intense vehicular traffic is recognized as a global societal problem, with a multifaceted influence on the quality of life of a person. Intelligent Transportation Systems (ITS) can play an important role in combating such problem, decreasing pollution levels and, consequently, their negative effects. One of the goals of ITSs, in fact, is that of controlling traffic flows, measuring traffic states, providing vehicles with routes that globally pursue low pollution conditions. How such systems measure and enforce given traffic states has been at the center of multiple research efforts in the past few years. Although many different solutions have been proposed, very limited effort has been devoted to exploring the potential of social network analysis in such context. Social networks, in general, provide direct feedback from people and, as such, potentially very valuable information. A post that tells, for example, how a person feels about pollution at a given time in a given location, could be put to good use by an environment aware ITS aiming at minimizing contaminant emissions in residential areas. This work verifies the feasibility of using pollution related social network feeds into ITS operations. In particular, it concentrates on understanding how reliable such information is, producing an analysis that confronts over 1,500,000 posts and pollution data obtained from on-the- field sensors over a one-year span.Comment: 10 pages, 15 figures, Transaction Forma

Erythropoietin: Recent Developments in the Treatment of Spinal Cord Injury

Erythropoietin (EPO), originally identified for its critical function in regulating production and survival of erythrocytes, is a member of the type 1 cytokine superfamily. Recent studies have shown that EPO has cytoprotective effects in a wide variety of cells and tissues. Here is presented the analysis of EPO effects on spinal cord injury (SCI), considering both animal experiments concerning to mechanisms of neurodegeneration in SCI and EPO as a neuroprotective agent, and some evidences coming from ongoing clinical trials. The evidences underling that EPO could be a promising therapeutic agent in a variety of neurological insults, including trauma, are mounting. In particular, it is highlighted that administration of EPO or other recently generated EPO analogues such as asialo-EPO and carbamylated-EPO demonstrate interesting preclinical and clinical characteristics, rendering the evaluation of these tissue-protective agents imperative in human clinical trials. Moreover the demonstration of rhEPO and its analogues' broad neuroprotective effects in animal models of cord lesion and in human trial like stroke, should encourage scientists and clinicians to design clinical trials assessing the efficacy of these pharmacological compounds on SCI

Potential use of human adipose mesenchymal stromal cells for intervertebral disc regeneration: a preliminary study on biglycan-deficient murine model of chronic disc degeneration

INTRODUCTION: Biglycan is an important proteoglycan of the extracellular matrix of intervertebral disc (IVD), and its decrease with aging has been correlated with IVD degeneration. Biglycan deficient (Bgn(−/0)) mice lack this protein and undergo spontaneous IVD degeneration with aging, thus representing a valuable in vivo model for preliminary studies on therapies for human progressive IVD degeneration. The purpose of the present study was to assess the possible beneficial effects of adipose-derived stromal cells (ADSCs) implants in the Bgn(−/0) mouse model. METHODS: To evaluate ADSC implant efficacy, Bgn(−/0) mice were intradiscally (L1-L2) injected with 8x10(4) ADSCs at 16 months old, when mice exhibit severe and complete IVD degeneration, evident on both 7Tesla Magnetic Resonance Imaging (7TMRI) and histology. Placebo and ADSCs treated Bgn(−/0) mice were assessed by 7TMRI analysis up to 12 weeks post-transplantation. Mice were then sacrificed and implanted discs were analyzed by histology and immunohistochemistry for the presence of human cells and for the expression of biglycan and aggrecan in the IVD area. RESULTS: After in vivo treatment, 7TMRI revealed evident increase in signal intensity within the discs of mice that received ADSCs, while placebo treatment did not show any variation. Ultrastructural analyses demonstrated that human ADSC survival occurred in the injected discs up to 12 weeks after implant. These cells acquired a positive expression for biglycan, and this proteoglycan was specifically localized in human cells. Moreover, ADSC treatment resulted in a significant increase of aggrecan tissue levels. CONCLUSION: Overall, this work demonstrates that ADSC implant into degenerated disc of Bgn(−/0) mice ameliorates disc damage, promotes new expression of biglycan and increased levels of aggrecan. This suggests a potential benefit of ADSC implant in the treatment of chronic degenerative disc disease and prompts further studies in this field

Decellularized silk fibroin scaffold primed with adipose mesenchymal stromal cells improves wound healing in diabetic mice

Introduction: Silk fibroin (SF) scaffolds have been shown to be a suitable substrate for tissue engineering and to improve tissue regeneration when cellularized with mesenchymal stromal cells (MSCs). We here demonstrate, for the first time, that electrospun nanofibrous SF patches, cellularized with human adipose-derived MSCs (Ad-MSCs-SF) or decellularized (D-Ad- MSCs-SF) are effective in the treatment of skin wounds, improving skin regeneration in db/db diabetic mice. Methods: The conformational and structural analyses of SF and D-Ad-MSCs-SF patches were performed by scanning electron microscopy, confocal microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. Wounds were performed by a 5mm punch biopsy tool on the mouse\u2019s back. Ad-MSCs-SF and D-Ad-MSCs-SF patches were transplanted and the efficacy of treatments was assessed by measuring the wound closure area, by histological examination and by gene expression profile. We further investigated the in vitro angiogenic properties of Ad-MSCs-SF and D-Ad-MSCs-SF patches by affecting migration of human umbilical vein endothelial cells (HUVECs), keratinocytes (KCs) and dermal fibroblasts (DFs), through the aortic ring assay and, finally, by evaluating the release of angiogenic factors. Results: We found that Ad-MSCs adhere and grow on SF, maintaining their phenotypic mesenchymal profile and differentiation capacity. Conformational and structural analyses on SF and D-Ad- MSCs-SF samples, showed that sterilization, decellularization, freezing and storing did not affect the SF structure. When grafted in wounds of diabetic mice, both Ad-MSCs-SF and DAd- MSCs-SF significantly improved tissue regeneration, reducing the wound area respectively by 40% and 35%, within three days, completing the process in around 10 days compared to 15-17 days of controls. RT2 gene profile analysis of the wounds treated with Ad- MSCs-SF and D-Ad-MSCs-SF showed an increment of genes involved in angiogenesis and matrix remodelling. Finally, Ad-MSCs-SF and D-Ad-MSCs-SF co-cultured with HUVECs, DFs and KCs, preferentially enhanced the HUVECs\u2019 migration and the release of angiogenic factors stimulating microvessel outgrowth in the aortic ring assay. Conclusions: Our results highlight for the first time that D-Ad-MSCs-SF patches are almost as effective as Ad-MSCs-SF patches in the treatment of diabetic wounds, acting through a complex mechanism that involves stimulation of angiogenesis. Our data suggest a potential use of DAd- MSCs-SF patches in chronic diabetic ulcers in humans

Mesenchymal Stromal Cells Primed with Paclitaxel Provide a New Approach for Cancer Therapy

BACKGROUND: Mesenchymal stromal cells may represent an ideal candidate to deliver anti-cancer drugs. In a previous study, we demonstrated that exposure of mouse bone marrow derived stromal cells to Doxorubicin led them to acquire anti-proliferative potential towards co-cultured haematopoietic stem cells (HSCs). We thus hypothesized whether freshly isolated human bone marrow Mesenchymal stem cells (hMSCs) and mature murine stromal cells (SR4987 line) primed in vitro with anti-cancer drugs and then localized near cancer cells, could inhibit proliferation. METHODS AND PRINCIPAL FINDINGS: Paclitaxel (PTX) was used to prime culture of hMSCs and SR4987. Incorporation of PTX into hMSCs was studied by using FICT-labelled-PTX and analyzed by FACS and confocal microscopy. Release of PTX in culture medium by PTX primed hMSCs (hMSCsPTX) was investigated by HPLC. Culture of Endothelial cells (ECs) and aorta ring assay were used to test the anti-angiogenic activity of hMSCsPTX and PTX primed SR4987(SR4987PTX), while anti-tumor activity was tested in vitro on the proliferation of different tumor cell lines and in vivo by co-transplanting hMSCsPTX and SR4987PTX with cancer cells in mice. Nevertheless, despite a loss of cells due to chemo-induced apoptosis, both hMSCs and SR4987 were able to rapidly incorporate PTX and could slowly release PTX in the culture medium in a time dependent manner. PTX primed cells acquired a potent anti-tumor and anti-angiogenic activity in vitro that was dose dependent, and demonstrable by using their conditioned medium or by co-culture assay. Finally, hMSCsPTX and SR4987PTX co-injected with human cancer cells (DU145 and U87MG) and mouse melanoma cells (B16) in immunodeficient and in syngenic mice significantly delayed tumor takes and reduced tumor growth. CONCLUSIONS: These data demonstrate, for the first time, that without any genetic manipulation, mesenchymal stromal cells can uptake and subsequently slowly release PTX. This may lead to potential new tools to increase efficacy of cancer therapy

DMTs and Covid-19 severity in MS: a pooled analysis from Italy and France

We evaluated the effect of DMTs on Covid-19 severity in patients with MS, with a pooled-analysis of two large cohorts from Italy and France. The association of baseline characteristics and DMTs with Covid-19 severity was assessed by multivariate ordinal-logistic models and pooled by a fixed-effect meta-analysis. 1066 patients with MS from Italy and 721 from France were included. In the multivariate model, anti-CD20 therapies were significantly associated (OR = 2.05, 95%CI = 1.39–3.02, p < 0.001) with Covid-19 severity, whereas interferon indicated a decreased risk (OR = 0.42, 95%CI = 0.18–0.99, p = 0.047). This pooled-analysis confirms an increased risk of severe Covid-19 in patients on anti-CD20 therapies and supports the protective role of interferon

Some Remarks on Aesthetics and Computer Sciences

In this work, resorting to a literature review, we walk through the points of contact between two apparently far and distinct disciplines, computer science and aesthetics, with the aim of showing how one may instead provide theoret- ical grounding and new exciting research problems to the other and vice versa. Computer science, in fact, has evolved from being the discipline concerned with the design and implementation of hardware and software components, exclu- sively meant for the execution of computing tasks, to a multifaceted area of science which deals with information systems at large, including the study of the interfaces and algorithms capable of extending/interacting with the hu- man senses, perceptions and brain capabilities. Aesthetics, on the other hand, provides a well-established framework which may very well serve the purpose of analysing and discussing the existing and rising relationships among human beings, computer systems and the physical environment

Fast-FMS: Fast multimedia across 3G mobile networks

Fast-FMS is a new network protocol designed to support fast and effective multimedia delivery in 3G networks. In particular, Fast-FMS provides a reliable session between two mobile customers that want to transmit multimedia and data while on a voice conversation. The protocol leverages the multi-RAB feature of 2.5+ cellular networks to provide a new almost-real-time class of services that fill the gap between the costly circuit-switched video-call service and the basic services based on MMS. © 2005 IEEE

Using geosocial search for urban air pollution monitoring

While Twitter and other Online Social Networks (OSNs) or microblogs are considered as a source of information for breaking news or uproarious and unexpected events, they could also be exploited as a dense worldwide sensors network for physical measurements. The corpus of geotagged posts from OSNs includes people's feedbacks about a wide range of topics, with precise temporal and geographical metadata, that can be used as a support or an improvement to hardware sensors. For instance, if collocated people, independently and at the same time, write posts complaining about high temperatures, it could effectively denote a raise of heat in that place. In this paper, we explore the feasibility to use a geographical search on social networks, that is, a geosocial search, about air pollution related posts, as effective air impureness measurements. We evaluate our assumption in large cities over three continents of the planet, where a minimum increment about the number of air pollution related posts in an area, indeed corresponds to a raise of minimum pollution values in such area. Such a correlation can be exploited to integrate and extend existing air pollution monitoring networks. At the end of the manuscript we propose to further employ the time series of posts returned by the geosocial search to predict next pollution values